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KMID : 0364820150510040382
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Korean Journal of Microbiology
2015 Volume.51 No. 4 p.382 ~ p.388
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Isolation and identification of protease-producing bacteria from the intertidal zone in Jeju Island, Korea
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Moon Yonng-Gun
Subramanian Dharaneedharan
Kim Dong-Hwi
Park So-Hyun
Heo Moon-Soo
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Abstract
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Eleven protease-producing bacteria were isolated from the organisms’ external shells and the inorganic materials collected from intertidal zone of Jeju Island, Republic of Korea. The samples were diluted serially, inoculated on Zobell agar plates with 1% skim milk and incubated at 20°C. Clear zone forming colonies were selected as protease-producing bacteria and each strain was identified based on the phylogenetic analysis with their 16S rDNA sequences. Strains JJM125, JJM129, YG47 and YG49 belong to the marine bacterial genus Pseudoalteromonas; strain JJM122 belong to the genus Microbulbifer; strains YG51, YG52, YG62 and YG63 belong to the genus Vibrio; and strain YG65 belong to genus Bacillus. Hence, the present study suggests that these protease producing bacteria could be further used to develop new varieties of protease with various biotechnological applications.
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KEYWORD
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bacteria, intertidal zone, Jeju Island, protease
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